Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto?Oligosaccharides

The galacto-oligosaccharide synthesis by ?-galactosidase from Bacillus circulans is described mathematically using three kinetic models of different complexity – a simple power law approach, an extended Michaelis Menten kinetics and, in particular, advanced, innovative mechanistic model for biocatalysis. Based on detailed experimental study, comprehensive reaction network identified, and reliable database provided. evaluation the studied performed Akaike information criterion, which allows assessment accuracy taking into account transferability.